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Live Probiotics Contents 6 Criteria for Cultured Drinks and Yogurts.

To ensure potency of live probiotics contents, safety and bioavailabilty, thereby achieving reliable therapeutic results, 6 factors must be considered for cultured drinks and yogurt probiotics:

1. Manufactured in a cGMP Facility

Live probiotic contents manufactured in cGMP (current Good Manufacturing Practices) certificated facility is a pre-requisite to achieve efficacy and potency, otherwise you risk consuming a contaminated product caused by undesirable bacteria.

A 1990 independent laboratory study found that nine out of ten brands of popular Lactobacillus acidophilus probiotics actually contained no acidophilus at all. All nine contained contaminants and other species of lactobacilli instead.

2. Proper storage and non-exposure to direct sunlight

Live probiotics contents are sensitive to moisture and sunlight. Their survivability depends very much on storage condition and exidation caused by excessive exposure to direct sunlight.

Only a glass bottle or food grade foiled sachet packing will keep out moisture. Plastic is permeable to varying degrees of moisture. Keeping moisture out and maintaining the appropriate state keeps the probiotics in efficacy state. Proper packaging ensures potency of live probiotics contents.

To prevent oxidation from sunlight, a dark or amber coloured glass bottle will prolong storage shelf life. If this is not done, don't be mystified if test reveals label claims on number of live, viable bacteria at the time of opening or consumption becomes unfulfilled. When this happens, it will be impossible for the remaining surviving strains to effectively colonize the gut. 

Live Probiotics Contents

3. Probiotics drinks and yogurt must be kept refrigerated at all times

The bacteria need to be stored in a fridge at the store and they must also be shipped in refrigerated trucks to the store. Heat quickly kills bacteria and even at room temperature they will become active and soon live out their life cycle – think of what happens if you leave yoghurt on the counter.

The best way to preserve bacterial potency is by keeping it cold at all times, until you’re ready to ingest it. Freeze drying is the best method of preserving the bacteria and for this to be maintained, they must be kept very cold at all times until you’re ready to ingest them. Of course, keep your probiotics refrigerated at home too to ensure potency of live probiotics contents.

4. Strain and number of bacteria per serving must be listed on the bottle

Only certain strains of bacteria are potent and effective (eg. L. bulgaricus DDS-14 is excellent, L. bulgaricus DDS-13 is useless – remember, ‘bulgaricus’ is the species, ‘DDS-14′ is the strain). If the manufacturer just lists ‘acidophilus’ for example, chances are they’ve used a cheap and ineffective strain of L. acidophilus. There are approximately 200 identified strains of L. acidophilus, of these, only 13 have good antibiotic (against bad bacteria) qualities, thus, the strain is indeed very important.They must also list the number of bacteria guaranteed per serving (and this should be between 2-5 billion colony forming units per serving) at the time of opening.

This guarantee is key to potency of live probiotics contents: If a manufacturer only guarantees the number of bacteria at the time of manufacture, this is meaningless. At manufacture, a bottle may contain 2 billion cfu (colony forming units) per serving – however, by the time that bottle gets to you, the bacteria may be mostly dead.

Another trick to watch out for is that manufacturers will list (for example) two different strains of L. acidophilus, one of which is a good strain and the other a cheap useless one. Then they will list the total guaranteed bacteria per serving at 2 billion cfu. However, they haven’t told you how much of that total count is the effective strain and how much is the ineffective one. It’s also a good idea to use human strains (vs. bovine or porcine strains) for safety’s sake.

5. Avoid centrifuged or filter-extracted bacteria

The cheapest way of extracting the bacteria from their growing culture is by centrifuge extraction. In centrifugal extraction, the bacteria (which are embedded/attached to their growth medium) are put in a centrifuge and whirled around with great force at high speed. This greatly damages the bacteria as they’re hurled against the walls of the centrifuge and many of them are left ruptured and useless.

However, the manufacturer can still put this damaged, ruptured bacteria in a bottle and include it in the guaranteed count per serving. Technically, you’ll still be ingesting that amount of bacteria, it just won’t do you any good.

In ultrafiltration extraction methods, the bacteria are pressed through a filter that removes the larger molecules of their growth medium. However, when bacteria are growing in their culture, they form into chains as they multiply.

Ultrafiltration results in the breakup of these chains, separates the bacteria from their beneficial growth medium (supernatant) and can also damage the bacteria themselves. The best method of preserving bacteria (and the most expensive) is to freeze dry it along with its growth medium. Many scientists maintain it’s best to consume bacteria along with it’s growth medium (also known as the substrate or supernatant) since this protects the bacteria from stomach acid and provides a ready food source for the bacteria to consume as they establish themselves in your gastrointestinal tract.

Also, as the bacteria grow in the culture (of milk or vegetable matter), the growth and culturing process produces valuable substances such as vitamins, antioxidants, immune system factors, antimicrobial compounds and digestive enzymes that greatly benefit your body when ingested. Make sure your brand of bacteria states on the bottle that it doesn’t use ultrafiltration or centrifugal extraction methods (if it doesn’t say so, chances are it does use these methods) and ideally, purchase bacteria that is freeze dried along with its growth medium.

6. Avoid prebiotics

Some companies package their live probiotics contents with fructooligosaccharides (FOS) and/or inulin – indigestible substances referred to as prebiotics, which they claim feed the bacteria, thereby improving performance. Keep in mind though, that many bacteria (both good and bad) can feed on these substances.

So if you have a predominantly bad bacterial flora (as most, if not all people with IBD do) consuming prebiotics may exacerbate your symptoms. Also, most FOS is manufactured via chemical synthesis and in many instances has been shown to cause abdominal pain, bloating and gas. I especially don’t recommend it for people with IBS or IBD. Also avoid FOS and inulin in vitamin/mineral supplements, whey protein powders, etc. Be sure to read labels as it’s become popular to add it to all kinds of products. For perfectly healthy people with an established good bacterial flora, prebiotics are probably okay, especially if they are not able to obtain these substances naturally through a good diet.

Health Canada put out a pretty good discussion paper on live probiotics contents, which addressed some of these concerns about most probiotics not being manufactured or stored properly to ensure potency of live probiotics contents. Here are just a few highlights from that paper:

Impact of processing, environment and diet on probiotic bacteria

“Probiotics in NHPs [natural health products] and foods are fragile and particularly sensitive to processing conditions (such as freezing, drying, exposure to oxygen) and to storage conditions (such as room temperature, oxygen, moisture).”

Quality Concerns

“A significant quality issue for probiotic supplements is the viability of bacteria in the product, specifically how many organisms are alive when the consumer purchases it as many products claim only the amount at the time of manufacture. As well, the product should contain the bacterial species that it claims on the label, and potentially pathogenic microorganisms should not be present.

A recent Canadian study assessed whether commercially prepared live probiotics contents products contained viable organisms, as claimed by the manufacturers, and particularly whether products labeled as containing Lactobacillus did so.(2) An additional objective of the research was to identify and quantify as many species as feasible and to compare them with the contents listed on labels. The design was randomized, double-blind trial of 10 brands of probiotic preparations bought over-the-counter in British Columbia ‘s lower mainland. Only products claiming to contain Lactobacillus were included in the study. The study measured the viable organisms in each probiotic brand and quantities of Lactobacillus in each product.

The results showed that none of the 10 products tested matched their labeled microbiologic specifications and two brands grew nothing aerobically or anaerobically. No Lactobacillus grew in five brands, although their labels stated that this was the main species. Eight brands contained viable cells, but only 10% of the number stated by their manufacturers. Most product labels did not adequately identify or quantify microbes which led the author to conclude that the use of live probiotics contents should not be recommended at this time.

In research conducted by a media outlet and broadcasted in 2003(3), two different probiotic capsules were each tested twice, including newly stocked retail product to determine the number of live bacteria and secondly near the end of the product’s shelf life to test if the counts had fallen. The first label claimed 6 billion live probiotics contents or cultures per capsule but testing showed only 1.7 billion and within two weeks, millions more bacteria died, with 460 million still alive. The second product did contain the label claim of more than one billion in the first test. But on the follow-up test, 692 million bacteria remained alive.

Dr. Gregor Reid, director, Canadian Research and Development Centre for Probiotics, Lawson Health Research Institute, and professor of Microbiology and Immunology, University of Western Ontario , London was quoted on the program as stating “This is particularly disheartening, as you’re getting a massive drop in viability, even within two weeks. You’ve picked two products but you could have picked 10 or 15 and, according to European studies, you’d find the same kind of results where you get a drop off in viability.”

Research has further shown that products should contain between one million and one billion live bacteria to be efficacious.(4) The media program concluded by stating that ” … manufacturers can say whatever they want on those containers. That’s because there are no Canadian government regulations on how much live probiotics contents there should be, and no rules on what labels have to tell us about quantity”.”


(1) “Lactobacillus and bifidobacterium in irritable bowel syndrome: Symptom responses and relationship to cytokine profiles” by Liam O’Mahony et al. Gastroenterology March 2005;128:541-551,783-785

(2) Huff, B.A. 2004. Caveat emptor. “Probiotics” might not be what they seem. Can. Fam. Physician. Apr. 50:583-7.

(3) CBC MARKETPLACE: PROBIOTICS. Testing bacteria levels. September 9, 2003. www.cbc.ca/consumers/market/ files/food/yogurt/

(4) Reid G, Jass J, Sebulsky MT , McCormick JK. 2003. Potential uses of probiotics in clinical practice. Clin. Microbiol. Rev. 16:658-672.

Posted on December 10, 2010 by JINI in Health and Gut Stuff

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